APPLICATION OF Aspergillus wentii ENZYME INCLEANING CRUDE OIL POLLUTED SOIL​

Microorganisms have a great metabolic diversity, which allows their ubiquity. Because of their ubiquitous nature, the biotechnological potential of microorganisms is virtually endless, with many possible applications. One of these applications is the utilization of microorganisms or their enzymes in petroleum bioremediation approaches. The contamination of the habitats by crude oil poses major public health and soclo-econornic hazards. Some fractions of crude oil are toxic to living organisms. The leakage of crude oil into soil damages the biota residing in the soil, including microorganisms and plants, and becomes increasingly toxic beyond a concentration. The extracted enzyme was added to the log of Polluted soil preparation in replicates as Rep 1, Rep2 and Rep3. Control sample was the polluted soil sample without addition of crude enzyme. Each sample was analyzed for residual hydrocarbon at 4 days interval for 12 days. The result is expressed in (mg/kg) in this research the cleaning of crude oil polluted environment using Aspergllius wentii was carried out on a polluted site. In the table above, day 0 has residual hydrocarbon of the fungi (Aspergillus wentii) presented on the table Rep 1, Rep 2 and Rep 3 has a sample of average 9442.82mg/kg and control of 9543. 62mg/kg. In day 4 the residual hydrocarbon of Rep 1, Rep 2 and Rep 3 on the table has a sample average of 5586.87mg/kg and a control of 8703.78 mg/kg In day 8, Rep 1, Rep 2 arid Rep 3 has sample average of 2670.42 mg/kg and control of 7937.85 mg/kg. In day 12, the residual hydrocarbon of the fungi Aspergillus wentii has a sample average of 1599.55 and control of 7239.32 mg/kg. The sample average has a standard deviation of 3509.53 mg/kg and the control of 992.1 7mg/kg.